Poster Presentation 31st Lorne Cancer Conference 2019

TotalSeq™, standardized oligo barcode antibody conjugates for highly-multiplexed immunophenotyping by single-cell sequencing (#179)

Kathryn Friend 1 , M Li 1 , K Nazor 1 , A Rahman 2 , B Yeung 1 , X Zhao 1 , X Yang 1 , M Stoeckius 3
  1. BioLegend, San Diego, California, USA
  2. Icahn School of Medicine at Mount Sinai, New York, USA
  3. New York Genome Center, New York, USA

CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is a recent advance in single-cell analysis, which leverages high-throughput single-cell sequencing (scSeq) to obtain measurements of both cellular proteins and transcriptomes. This platform is transforming how complex cell populations are studied. Published data, as well as those from our own studies, indicates that scSeq analysis on cell surface marker expression is comparable to multi-color flow cytometry, but provides superior multiplexing capabilities. To support this new technology and enable reliable comparison of data across longitudinal and multi-site studies, we provide standardized oligonucleotide barcode-labeled antibodies (TotalSeq™).  After assigning a unique oligo barcode to each of our monoclonal antibodies, we prepared the conjugated reagents.  In addition, we assigned/conjugated a series of oligonucleotide barcodes to select clones of antibodies specific to “universally” expressed cell surface molecules.  These products (Hashtags) can be used to label cells from different sources, experiments, etc., when pooling samples prior to single-cell compartmentalization.  We show our recent data in validating TotalSeq™ and the corresponding Hashtag products.